Recombinant La Crosse Virus,Envelope Glycoprotein 1 (LACV G1) anticorps

N° du produit ABIN7543088

Cet anticorps Souris Monoclonal détecte spécifiquement dans IP, Neut, ELISA, WB, FACS et Sero. Il présente une réactivité envers La Crosse Virus (LACV).

Aperçu rapide pour Recombinant La Crosse Virus,Envelope Glycoprotein 1 (LACV G1) anticorps (ABIN7543088)

Antigène: La Crosse Virus,Envelope Glycoprotein 1 (LACV G1)

Type d'anticorp: Recombinant Antibody

Reactivité: La Crosse Virus (LACV)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunoprecipitation (IP), Neutralization (Neut), ELISA, Western Blotting (WB), Flow Cytometry (FACS), Serological Studies (Sero)

Clone: 807-31

Détail du produit

Fonction: Anti-La Crosse virus, G1 envelope glycoprotein [807.31], Mouse IgG1, kappa

Specificité: This antibody is specific for the Bunyavirus La Crosse G1 viral glycoprotein. According to the original publication (Gonzalez-Scarano et al, 1982), this antibody clone 807.31 was validated to belong to Group A antibodies, which were virus specific and with high neutralization (N) and hemagglutination inhibition (HI) titers. La Crosse Virus is an arbovirus (a virus transmitted by insects), it is one of the most important mosquito-borne viruses in the United States. La Crosse virus contains two glycoproteins, G1 and G2, the larger of which, G1, is the target of neutralizing antibodies.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising BALB/c mice with La Crosse viruses and the harvested spleens were fused with mouse P3X63Ag8.653 myeloma cells.

Isotype: IgG1 kappa

Information d'application

Indications d'application: In the original publication, this antibody, together with other 22 clones against the the G1 and N proteins of LaCrosse and Tahyna, was characterised by the ELISA assays, immunoprecipitation, neutralisation tests, and hemagglutination inhibition tests (Gonzalez-Scarano et al, 1982). It was used for antigenic taxonomy of Califmia serogroup viruses and for the identification of the California serogroup viruses of North America (Gonzalez-Scarano et al, 1982). It was also used, together with the other anti-La Crosse mAb clones 807.35 and 807.27, in the neutralisation assays of murine leukemia virus pseudotypes of La Crosse and Hantaan Bunyaviruses to validate a system for analysis of cell tropism (Ma et al, 1999). Furthermore, a single immunisation with this antibody was reported to result in a robust immune response and protection against La Crosse virus (Pekosz et al, 1995). Recently, this antibody has been reported in various FACS analyses, for example, to demonstrate that mutagenesis of the La Crosse Virus glycoprotein supports a role for Gc (1066-1087) as the fusion peptide (Plassmeyer et al, 2007), and to suggest that the fusion peptide of La Crosse virus Gc is a determinant of properties associated with neurotoxicity (Soldan et al, 2010).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: La Crosse Virus,Envelope Glycoprotein 1 (LACV G1)

Autre désignation: La Crosse virus, G1 envelope glycoprotein

Classe de substances: Viral Protein

Sujet: LCV G1, G1 envelope glycoprotein, G1 glycoprotein, G1 protein, Envelopment polyprotein, M polyprotein, Glycoprotein G1

UniProt: Q8JPR1